Explanation
A screenshot of a computer Description automatically generated with low confidence

Box 1: Yes
User1 already has full control access through membership of Group1. Therefore, User1 can write to the file.
Box 2: No
The planned changes will grant User2 full control access to the file. However, User2 is a member of Group2 which has Deny/Write access. The Deny permission will always take precedence. Therefore, User2 will not be able to write to the file.
Box 3: No
The planned changes will grant User3 full control access to the folder. That permission will be inherited by the file. However, User3 is a member of Group2 which has Deny/Write access. The Deny permission will always take precedence. Therefore, User3 will not be able to write to the file.